Erythrocyte-derived microvesicles induce arterial spasms in JAK2V617F myeloproliferative neoplasm

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JAK2V617F mRNA metabolism in myeloproliferative neoplasm cell lines

Myeloproliferative neoplasms (MPNs) are a heterogeneous group of leukemias with defective regulation of myeloid stem cell proliferation. They include four distinct diseases: chronic myeloid leukemia, polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). In 2005, four independent studies have concurred to the identification in MPN patients of a specific mutation...

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Evaluation of JAK2V617F mutation prevalence in myeloproliferative neoplasm by AS-RT-PCR

Abstract Objective JAK2 is a non-receptor tyrosine kinase that plays a major role in myeloid disorders. JAK2V617F mutation is characterized by a G to T transverse at nucleotide 1849 in exon 12 of the JAK2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the JAK2 protein. Methods In this study we evaluated RNA from 89 pati...

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Jak2V617F myeloproliferative neoplasm stem cells and interferon-alpha

The myeloproliferative neoplasms (MPNs) are clonal disorders of hematopoiesis that arise as a result of aberrant activation of tyrosine kinases and result in the proliferation and accumulation of mature myeloid cells in the blood, bone marrow and spleen. The prototypical MPN, chronic myeloid leukemia (CML) is caused by constitutive activation of ABL kinase occurring as a result of the BCR-ABL f...

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Detection of the JAK2V617F mutation with the Ipsogen MutaScreen kit: absence of JAK2V617F does not mean absence of myeloproliferative neoplasm.

Michael R. Lieber Norris Comprehensive Cancer Center and Departments of Pathology, Biochemistry & Molecular Biology, Molecular Microbiology & Immunology, and Biological Sciences (Section of Molecular and Computational Biology), University of Southern California Keck School of Medicine, Los Angeles, CA Contribution: A.G.T. designed and performed research and wrote the manuscript; A.Y. designed a...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 2020

ISSN: 0021-9738,1558-8238

DOI: 10.1172/jci124566